Plant Transcription Factor Database
Previous version: v3.0
Capsella rubella
FAR1 Family
Species TF ID Description
Carubv10000250mFAR1 family protein
Carubv10000255mFAR1 family protein
Carubv10000262mFAR1 family protein
Carubv10004178mFAR1 family protein
Carubv10004190mFAR1 family protein
Carubv10007256mFAR1 family protein
Carubv10007820mFAR1 family protein
Carubv10008456mFAR1 family protein
Carubv10012599mFAR1 family protein
Carubv10012703mFAR1 family protein
Carubv10013009mFAR1 family protein
Carubv10013017mFAR1 family protein
Carubv10013031mFAR1 family protein
Carubv10014621mFAR1 family protein
Carubv10017836mFAR1 family protein
Carubv10018400mFAR1 family protein
Carubv10019871mFAR1 family protein
Carubv10019873mFAR1 family protein
Carubv10022618mFAR1 family protein
Carubv10022651mFAR1 family protein
Carubv10024069mFAR1 family protein
Carubv10028009mFAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885